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KMID : 0380020070220040218
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Korean Journal of Biotechnology and Bioengineering
2007 Volume.22 No. 4 p.218 ~ p.221
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Octimization of Score Production via Sonication of Antifungal Polyene-producing Actinomycetes
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Han Kyu-Boem
Kim Eung-Soo
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Abstract
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The polyene antifungal antibiotics, mostly produced by Gram-positive soil actinomycetes, are a family of type I polyketide macrolide ring compounds with 2040 carbon backbone contain 38 conjugated double bonds. Using polyene-specific genomic screening strategy, we previously isolated three novel polyene-producing actinomycetes strains from soil, implying the potential application of these strains’ spores as microbial pesticides. Here, we report that the sonication is a very efficient method for actinomycetes spore generation with a sonicator power-dependent manner. In addition, these sonication-driven actinomycetes spores retained significant portion of their cell viabilities as well as antifungal activities after freeze-drying procedure, implying the potential application of these strains’ spores as microbial pesticides.
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KEYWORD
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Spore production, polyene, actinomycetes, sonication
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